A compound microscope is an instrument used to view objects in the laboratory. Its main use is to make the objects that are not observable with the naked eye be visible using the microscope. We have the electronic type of microscope that gives finer details of an object to be viewed. It’s common in the biological laboratory. Compound microscopy is the use of a compound microscope to view the objects parts such as cell membranes.
A compound microscope consist of the following parts: eye piece lens, objective lens, condenser lens, course adjustment knob, fine adjustment knob, tube, arm, base, illuminator (mirror), stage, revolving nosepiece, rack stop, iris, inclination joint and body tube.
It’s an instrument handled with care during the use. Skill personnel are required to carry out the operations. Focus the lower power objective lens while checking from sides, the lens should be close to the specimen without being in contact. Using the eyepiece lens focus upward until the image is focused. Use the lower power lens to click in the power lens. Use the fine adjustment knobs to carry out the minor adjustments.
The general importance of magnification of microbiological organism is to enable us to understand the organism while seeing them with our naked eye. The microorganisms are very tiny to an extend we are not in apposition to notice their presence. They may be positive or negative, both affect the living organisms. For example, presence of germs in water may result into a disease outbreak like cholera. For the treatment to be done we have to analyze the water to know what causes the outbreak therefore a need to use microscope.
Compound microscope was invented by Zacharias Janssen around 1590. Its effective and convenient use is concentrated in the power of magnification. The more the magnification capacity the more the details can be seen. The major parts used include eyepiece and objective lens to give the magnification given in terms multiplication of a number e.g. 10x.
Compound microscope consists of the following parts and their functions explained. The eyepiece lens has a lens on top, used for viewing the specimen. The body tube connects the objective lens to eyepiece lens. It’s always larger in circular parts. The arm of a microscope is to give support i.e. it’s join the tube and the base. The base is the bottom most part of a compound microscope. The base gives a support for the whole instrument. An illuminator assists in provision of lights from the surrounding environment up passing through stage bottom.
Mirror can be used in this case but a steady light source should be taken into consideration for more visibility and accurate results. A stage is a position where the slides are placed. The slides are pieces of rectangular cut glasses used for placement of the specimen. The slides are dip into water before the specimen is placed into them to allow for the visibility. The slides are transparent to allow the lights to pass through them for clear observation. Use the stage clips to hold the slides into position. In case the slides are not placed into position use the turning knobs to move them either right or left or moving the slides up and down depending with the required position.
The resolving nosepiece is used to hold the objectives lenses and rotated to change the resolution power more easily. An objective lens varies from shortest or longest, shorter give less power while longer lens gives more power. They have power resolutions to give more details of the specimen depending with the resolution power. A rack stop adjustment used to determine how close the object lens is with the slide. Most manufactures set it while still in the factory to avoid the cranking high power objective lens. A condenser lens is used to focus the light into the specimen. They most effective at high power i.e. 400x and above. The diaphragm consist of different size holes, it’s used to vary the intensity and size of the cone of light focused upwards into the slide. The setting of the iris depends on the degree of the operator.
In conclusion, compound microscopy is essential in our daily life. The use of the machine enables us to carry out self-awareness to the community regarding basic hygiene conditions like washing our hands with detergents before we eat our food. Moreover, we use the microscope to understand our body parts and functions more effectively such as membrane.